Plasmids And Cloning Vectors ppts

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project researchers to copy genes and other pieces of chromosomes to generate enough identical ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 18, 2016 - Views: 1

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No Slide Title

Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication, determines the number of copies per cell Marker genes: ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: August 27, 2016 - Views: 1

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: August 19, 2016 - Views: 1

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16.2 In vivo gene cloning – the use of vectors -...

16.2 In vivo gene cloning – the use of vectors. The importance of ‘sticky ends’. ... allowing the plasmids to pass through, into the cell. Calcium ion medium.

http://vbio.weebly.com/uploads/2/8/2/4/2824362/16.2_-_in_vivo_gene_cloning_-_the_use_of_vectors.pptx

Date added: August 19, 2016 - Views: 1

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Molecular Cloning Methods - Santa Monica College

Title: Molecular Cloning Methods Author: Santa Monica College Last modified by: Santa Monica College Created Date: 10/16/2001 2:16:45 AM Document presentation format

http://homepage.smc.edu/bober_mary/Bio%2022/ch%2018%20recombinant%20DNA%20technology%20%20lecture%20darwin%2006%20part%201%20and%20part%202.ppt

Date added: August 18, 2016 - Views: 1

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Plasmid - ffarmasi.unand.ac.id

vectors. Plasmids serve as important tools in genetics and biotechnology labs, ... Other types of plasmids are often related to yeast cloning vectors that include:

http://ffarmasi.unand.ac.id/bahanajar,rpkps,jurnal,buku,cv/BA.RPKPS/marlina/Biotek/Plasmid,%202014.pptx

Date added: August 21, 2016 - Views: 1

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain plasmid Types of Genes No Lactose Present Lactose Present ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: August 20, 2016 - Views: 1

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Plasmid - KSU Faculty

Some of plasmid applications. Plasmids serve as important tools in genetics and ... 3 Molecular cloning. Plasmids used in genetic engineering are called . vectors.

http://fac.ksu.edu.sa/sites/default/files/plasmid_lab_.pptx

Date added: August 19, 2016 - Views: 1

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... A. Bacterial Vectors. 1. Plasmids. 2. Bacteriophage. ... Plant Cloning Vectors. 4.

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: August 18, 2016 - Views: 1

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CHAPTER 4 DNA CLONING -...

The vectors commonly used in gene cloning were originally derived from two natural sources. 1. Plasmids. 2. Viruses. CLONING VECTOR: PLASMID. CHARACTERISTICS.

https://aliazamani.files.wordpress.com/2015/07/lec-4-gene-cloning.pptx

Date added: August 19, 2016 - Views: 1

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Chapter 13 An Introduction to Cloning and...

Chapter 13 An Introduction to Cloning and Recombinant DNA Clones Genetically identical organisms or molecules derived from a common ancestor Cloning Plants from ...

http://www.bio.brandeis.edu/classes/heredity/Lecture%20Powerpoints/Chapter_13_1.ppt

Date added: August 19, 2016 - Views: 1

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Chapter 9 – DNA-Based Information Technologies

Chapter 9 – DNA-Based Information ... cut directly across both strands leaving all dsDNA Cloning Vectors ... Cutting with EcoRI Cloning vectors can be: plasmids, ...

http://ww2.valdosta.edu/~dgosnell/3601/May%202005/DNA-Based%20Information%20Technologies.ppt

Date added: August 21, 2016 - Views: 1

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Plasmid Isolation - site.iugaza.edu.ps

Isolation of Plasmid DNA ... are capable of surviving in the presence of the antibiotic kanamycin Molecular Biology Applications for Plasmids I. Cloning of DNA ...

http://site.iugaza.edu.ps/mzaid/files/2010/02/How-to-purify-plasmid-DNA-using-silica-based-columns1.ppt

Date added: August 27, 2016 - Views: 1

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Biotechnology and Recombinant DNA - Wolfweb...

Biotechnology and Recombinant DNA Chapter 9 I. Learning Objectives Why genetic engineering? Cloning basics Beyond the basics Overview of DNA manipulation II.

http://wolfweb.unr.edu/homepage/borgeson/Bio251/10biotechnewa.ppt

Date added: August 18, 2016 - Views: 1

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Library construction - cbcb.umd.edu

A plasmid vector for cloning Plasmid vectors Expression vector How to clone DNA ... Blue/White Color Screening Selecting Colonies with Recombinant Plasmids ...

http://www.cbcb.umd.edu/confcour/CMSC828H-materials/Cloning-into-plasmids.ppt

Date added: August 26, 2016 - Views: 1

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Recombinant DNA Technology & Cloning

Multiple Cloning Sites. Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases ...

https://lectureug4.files.wordpress.com/2012/03/biotech-lect-2.pptx

Date added: August 19, 2016 - Views: 1

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Introduction to Vectors - The Pingry School

Plasmid Engineering p. 1-2 Cloning Scheme Digest ... Introduction to Vectors ... Slide 2 Slide 3 Slide 4 Plasmids Vectors Plasmid Engineering ...

http://faculty.pingry.org/domara/documents/WAKSMANLECTURE1_sept09.ppt

Date added: August 19, 2016 - Views: 1

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Inquiry into Life Twelfth Edition - Regis...

Summary Basic Plasmid Cloning Vectors. pBR322 and the pUC plasmids. pBR322 has . 2 antibiotic resistance genes . ... Inquiry into Life Twelfth Edition Last modified by:

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 21, 2016 - Views: 1

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DNA Technology & Gene Mapping - Mr. Kelly's Home...

DNA Cloning. Most techniques for cloning make use of bacteria (E. coli) as they have small circular plasmids that are receptive for insertion of foreign DNA.

http://www.mrkelly.santasusana.org/DNA%20Technology%20&%20Gene%20Mapping.pptx

Date added: August 26, 2016 - Views: 1

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DNA Technology - Quia

DNA technology. has rapidly ... have plasmids and phages available for use as gene-cloning vectors, ... have plasmids that can be used as gene vectors, and.

https://www.quia.com/files/quia/users/pbruce1/DNA12

Date added: August 18, 2016 - Views: 2

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PowerPoint Presentation

* Cloning vectors * Cloning vectors In gene ... most commonly used are plasmid cloning vectors. * Plasmids Extra-chromosomal DNA found in ... PowerPoint Presentation ...

http://www.educationscotland.gov.uk/Images/Background_Transformation_PowerPoint_H_tcm4-664851.ppt

Date added: August 20, 2016 - Views: 1

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PowerPoint Presentation

Cloning site Cloning Genes Plasmid vectors Linkers: synthetic DNA fragments containing restriction sites Transformation of host cells with plasmids Selectable ...

http://biology.illinoisstate.edu/wanicho/bsc220/ppt/cloning%20and%20transformation.ppt

Date added: August 27, 2016 - Views: 1

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CHAPTER 20 – DNA Technology and Genomics

Bacterial plasmids are widely used as cloning vectors because they can be isolated from bacteria, manipulated to form recombinant plasmids by . in vitro.

http://www.cbsd.org/cms/lib010/PA01916442/Centricity/Domain/2102/Chapter%2020%20DNA%20Tools%20and%20Biotech%2010.pptx

Date added: August 19, 2016 - Views: 1

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PowerPoint Presentation

Cloning Vectors Enable DNA molecules to be replicated inside host ... Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2.

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: August 27, 2016 - Views: 1

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PowerPoint Presentation

DNA-based information technologies Understand basics steps/enzymes/features of DNA cloning Know main types of cloning vectors used - pros & cons of each

http://chemistry.creighton.edu/%7Ejksoukup/DNAtech2_2010.ppt

Date added: August 20, 2016 - Views: 1

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Ch. 20 DNA Technology and Genomics -...

Use bacterial plasmids (cloning vector). ... as vectors helps avoid gene expression problems. ... Ch. 20 DNA Technology and Genomics Last modified by:

https://www.tracy.k12.ca.us/sites/jhaut/Documents/AP_Biology/lecture%20notes/Ch.%2020%20biotech/Ch.%2020%20Biotechnology%20notes-2012.pptx

Date added: August 19, 2016 - Views: 1

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DNA Technology - NCSSM Links

DNA Technology Overview DNA ... DNA vectors, and host organisms Either plasmids or bacteriophages can ... gene cloning results when the foreign genes replicate inside ...

http://courses.ncssm.edu/mccoy/DNA%20Technology.ppt

Date added: August 18, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 4500/5500 ... gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Lecture%203.ppt

Date added: August 27, 2016 - Views: 1

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Steps in Gene Cloning : The use of pBR322 as...

pUC plasmids are based on pBR322 – having MCS. The procedure shown seeks to clone the human β-globingene into a plasmid ... Other phage vectors used in gene cloning.

https://aliazamani.files.wordpress.com/2015/07/lec-4-gene-cloning-cont.pptx

Date added: August 27, 2016 - Views: 1

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Recombinant Baculovirus: - Ohio University

Recombinant Baculovirus as a Highly Potent Vector for Gene Therapy of Human Colorectal Carcinoma: Molecular Cloning, ... Two Plasmids Used.

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/Jenna%20Kausner.pptx

Date added: August 19, 2016 - Views: 1

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PowerPoint Presentation

Plasmids are classified 1. by their ability to be transferred to other bacteria. ... The Cadillac of Cloning Vectors. pBR322. Clone fragment in one antibiotic gene.

http://docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/4-plasmidi.pptx/at_download/file

Date added: August 27, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1; (2) bla ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture211.ppt

Date added: August 27, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Complementary DNA or cDNA cloning: ... make and screen gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: August 18, 2016 - Views: 2

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Bacterial recombination- Transduction - MCCC

Why do we care about homologous recombination? ... Plasmids can be cloning vectors Ch 8 pg 175 Plasmid requirements in biotech PowerPoint Presentation ...

http://www.mccc.edu/~blinderl/documents/Ch15_bacteriaslide_set2.ppt

Date added: August 27, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Cloning vectors Eukaryotic gene ... Bacteria carrying recombinant plasmids therefore give rise to white colonies. pGEM-3Z Cloning foreign DNA into a plasmid vector ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB720Lecture209.ppt

Date added: August 27, 2016 - Views: 1

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PowerPoint Presentation

DNA ligase splices pieces into plasmids or other cloning vectors How to form recombinant DNA?

http://skywalker.cochise.edu/ashraft/web/BIO101ppt/What%20is%20biotechnology.ppt

Date added: August 27, 2016 - Views: 1

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VOCABULARY for GENETIC TECHNOLOGY

VOCABULARY for GENETIC TECHNOLOGY CLONING Produces an ... Plasmids Plasmids are small rings of DNA in bacteria ... Transgenic organisms Plasmids VECTORS ...

http://www.bville.org/tfiles/folder196/VOCABULARY%20for%20GENETIC%20TECHNOLOGY%2Eppt

Date added: August 27, 2016 - Views: 1

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Ch. 9: Presentation Slides

The simplest types of vectors are plasmids whose DNA is double-stranded and circular. ... Cloning Vectors. ... Ch. 9: Presentation Slides

http:[email protected]3A6B.pptx

Date added: August 20, 2016 - Views: 1

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Microbial Genetics - ALRlab

Microbial Genetics WHY? terms Genotype ... steps in cloning Restriction-modification enzymes “shot gun” or PCR Ligase Recombination Plasmids (or phage)= vectors ...

http://alrlab.pdx.edu/media/Lec12.ppt

Date added: August 19, 2016 - Views: 1

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Chapter 9 Molecular Genetic Techniques and...

Chapter 9 Molecular Genetic Techniques and Genomics Presented by: Troy Coke What and Why? What?: A gene of interest is inserted into another organism, enabling it to ...

http://www.metabolism.net/bidlack/cellphys/doc/powerpoint%20s2004/Recombinant%20DNA.ppt

Date added: August 27, 2016 - Views: 1

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PowerPoint Presentation

Types of cloning vectors Plasmids: Autonomously replicating circular DNA molecules. 2. Bacteriophage: Small viruses that infect bacteria cells. Vectors for large DNA ...

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: August 27, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 450/550 ... gene libraries How to make and screen cDNA libraries Several different cloning vectors including plasmids, ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: August 27, 2016 - Views: 1

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No Slide Title

... Restriction enzymes Cloning Vectors Plasmids that can be modified to carry new genes Plasmids useful as cloning vectors must have a replicator (origin of ...

http://faperta.ugm.ac.id/download/pak_tar/genetika-molekuler/cloning20061.ppt

Date added: August 27, 2016 - Views: 1

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Genetic Modification of Organisms - IWS.COLLIN.EDU

Gene cloning. leads to the ... are often used as vectors. 0. ... These are organisms that have had foreign genes inserted into their genome through plasmids created ...

http://iws.collin.edu/kflake/Biology1408/Ch%2012S.pptx

Date added: August 18, 2016 - Views: 1

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Viral Vector Training - safe.rbhs.rutgers.edu

Viral Vectors . Can be used as gene ... Limited cloning capacity. ... Spread the genomes of the helper plasmid into multiple plasmids which would require . multiple.

https://safe.rbhs.rutgers.edu/~umd_apps/eohss/viral_vector/viral_vector.ppsx

Date added: August 21, 2016 - Views: 1

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ISOLATION OF DNA - Iroquois Central School...

Plasmids. Plasmids are small, circular, extrachromosomal DNA molecules found in bacteria, which can. replicate on their own, outside of a host cell.

http://www.iroquoiscsd.org/cms/lib/NY19000365/Centricity/Domain/112/AP%20BI3.pptx

Date added: August 21, 2016 - Views: 1

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Recombinant DNA - mydsn.net

Chapter 7 Recombinant DNA Technology and Genomics

http://mydsn.net/docs-tools/BIO205/3rdIng07.ppt

Date added: August 18, 2016 - Views: 1

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Chapter 16.1

... in bacteria that carry only a few genes and can replicate independently of the single “main” chromosome Cloning Vectors Cloning vectors: modified plasmids ...

http://www.rjwsciencestm.com/AP-Bio/Toolkit.ppt

Date added: August 23, 2016 - Views: 1

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Chapter 13

Cloning Vectors. Clone—an exact ... Plasmids serve as excellent vectors for DNA. Steps: Isolate the plasmid . from a bacteria cell . ... Chapter 13 Last modified by:

http://www.sasd.us/cms/lib6/PA01000821/Centricity/Domain/116/Chapter%2013%20Gene%20Technology.pptx

Date added: August 20, 2016 - Views: 1